Leica Patio Umbrella NCL L ALK User Manual

NovocastraLiquid  
Mouse Monoclonal Antibody  
p80 (Anaplastic Lymphoma Kinase)  
Product Code: NCL-L-ALK  
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.  
Intended Use  
Specificity  
Clone  
Human ALK protein and C-terminal ALK fusion proteins containing the immuized target sequence.  
5A4  
IgG1  
Ig Class  
Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase  
catalytic domain and part of the C-terminus of the NPM-ALK transcript (419-520 amino acids).  
Antigen Used for  
Immunizations  
Mouse myeloma (p3-NS1-Ag4-1).  
Hybridoma Partner  
Liquid tissue culture supernatant containing sodium azide.  
Volume as indicated on vial label.  
Preparation  
Not evaluated  
Yes  
Effective on Frozen Tissue  
Effective on Paraffin Wax  
Embedded Tissue  
Immunohistochemistry on paraffin sections.  
Recommendations on Use  
Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra  
Epitope Retrieval Solution pH 6.  
Suggested dilution: 1:200 for 30 minutes at 25 °C. This is provided as a guide and users should  
determine their own optimal working dilutions.  
Visualization: Please follow the instructions for use in the NovolinkPolymer Detection Systems.  
For further product information or support, contact your local distributor or regional office of Leica  
The performance of this antibody should be validated when utilized with other manual staining  
systems or automated platforms.  
Western Blotting: Not recommended  
Immunohistochemistry: a proportion of anaplastic large cell lymphomas.  
Cytoplasmic and/or nuclear.  
Positive Controls  
Staining Pattern  
Store at 2–8 °C. Do not freeze. Return to 2–8 °C immediately after use. Do not use after  
expiration date indicated on the vial label. Storage conditions other than those specified above  
must be verified by the user.  
Storage and Stability  
This reagent has been prepared from the supernatant of cell culture. As it is a biological product,  
reasonable care should be taken when handling it.  
Warnings and Precautions  
This reagent contains sodium azide. A Material Safety Data Sheet is available upon request or  
p80 is encoded by a hybrid gene that occurs as a result of the chromosomal translocation, t(2;5)  
(p23;q35). The protein consists of the amino terminus of the nucleophosmin (NPM) gene fused  
to the catalytic domain of anaplastic lymphoma kinase (ALK). Full-length ALK is a 1620 amino  
acid protein that exhibits 57% amino acid identity to leukocyte tyrosine kinase (LTK). The mature  
form of ALK is a 200 kD glycosylated single chain molecule that occurs on the surface of cell  
membranes and probably acts as a ligand receptor.  
General Overview  
Leica Biosystems Newcastle Ltd  
Balliol Business Park West  
Benton Lane  
Newcastle Upon Tyne NE12 8EW  
United Kingdom  
(
+44 191 215 4242  
ALK-L 13/12/2012  
 
© Leica Biosystems Newcastle Ltd. NCL-L Rev D  

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